Name: Gergana Ganeva ZAHMANOVA

Date and place of birth: February 9, 1975, Stara Zagora, Bulgaria

Nationality: Bulgarian

Education: University of Plovdiv, Biological Faculty, Bulgaria.

Degrees: BS and MSc in Biology, PhD

Position: Assistant professor in Molecular Biology

 

 

Work address:

Department of Plant Physiology and Molecular Biology

University of Plovdiv

24, Tzar Assen Str.

4000 – Plovdiv

Telephone :+35932620944

E mail: gerganaz@uni-plovdiv.bg; gerganaz@gmail.com

Education:

2009

PhD Thesis: "Development of transgenic potato plants expressing Hepatitis B genes"

1994 – 1999

MSc degree in Biology, Specialization in Biotechnology, University of Plovdiv, Bulgaria; MSc Thesis: Development of transgenic potato plants resistant to herbicide "Basta"

Courses taught and other services provided to students at the home institution:

Since 2010

Theoretical courses of Molecular biology.

Since 2008

Practical courses of Gene engendering, Molecular genetics, Molecular ecology, Plant in vitro culture, Molecular biology.

International fellowships and courses:

2004 - Socrates/Erazmus Programme, "Aristotle University of Thessaloniki", Departament of  Genetics, Development and Molecular biology, Thessaloniki, Greece, Project " Molecular gene markers", Prof. Zacharias Scouras.

2009 - STSM, (COST ACTION FA0804 Molecular farming: plants as a production platform for high value protein), Project title: Expression of HBcAg-AIV chimaeras in plants using CPMV-HT technology, Hosting laboratory: John Innes Centre, Norwich, UK, Prof. George Lomonossoff.

2010 – Research visitor, John Innes Centre, Norwich, UK, Project title: Expression of antigens in plants, extraction and purification. BioSupport project fellowship

 

2011 – Research visitor, John Innes Centre, Norwich, UK

Languages:

English – writing, spiking, and understanding – very well

Russian – writing, spiking, understanding –well

Research experience:

• Plant In vitro culture and plant transformation (Agrobacterium – mediate gene transfer)

• basic techniques for DNA manipulation (gel electrophoresis of nucleic acids, nucleic acid radiolabelling (32P), Southern blots, molecular cloning and subcloning, restriction map analysis, DNA sequencing);

• basic techniques for protein molecular biology (SDS- PAGE of proteins, Western blots, fusion proteins expression, protein purification)

• computer methods for molecular analyses

• Polymerase Chain Reaction (PCR)

• Enzyme-linked immunosorbent assay (ELISA)

Scientific interests: Plant based vaccine, Edible vaccine, protein expression

Participation at meetings and courses:

September 4-5 2002, First conference of biological, ethical and ecological consequences of the extensive application of Genetically Modified Organisms "GMO – Future and Problems" Plovdiv Bulgaria, oral presentation "Transformation of potato plants with "core" protein gene from hepatitis B virus"

September 23-26 2004, International conference "Days of virology", oral presentation "Edible vaccine"

June 18-20 2007 "Plant Based Vaccines and Antibodies" PBVA, University of Verona, Verona Italy, Poster presentation "Development of HBV edible vaccine"

April 16-18, 2008 "Co-Extra training tool - Perspectives in coexistence and traceability along the supply chain: GMO detection, identification and quantification", ABI, Sofia, Bulgaria

September 24-27, 2008, International conference "Plant GEM" Poster presentation, Albena, Bulgaria

June 15-17, 2009 "Plant Based Vaccines and Antibodies" PBVA, University of Verona, Verona Italy

April 7 – 11, 2010 "4th European congress of Virology" Villa Erba, Cernobbio, Italy

October 13-15, 2010 "Modern vaccines and adjuvant" Cannes, France

 

Publications:

Oral immunization with Hepatitis B virus nucleocapsid expressed in transgenic potatoes; Gergana Zahmanova, Darryl Falzarano, Samir Naimov, Milena Kostova, Stefan Dukiandjiev, Ivan Minkov, and Anton Andonov, Proceedings of the Bulgarian Academy of Sciences (2008), 61(10) 1295-1302

Investigation of immunogenecity and stability of synthetic HBc 153 protein and WHV153 in human digestive tract; Gergana Zahmanova, Samir Naimov, Stefan Dukiandjiev, Ivan Minkov and Anton Andonov, Homo 2007, 40, 33-40

Expression of synthetic SN19 gene in transgenic potato; Samir Naimov, Gergana Zahmanova, Rumyana Boncheva, Milena Kostova, Ivan Minkov, Stefan Dukiandjiev, and Ruud deMaagd, Biotechnology and biotechnology equipment 2006, 3: 38-41

Molecular farming – the production of recombinant subunit hepatitis B vaccine in tobacco plants; Gergana Zahmanova, Stefan Dukiandjiev, Ivan Minkov and Anton Andonov, Proceeding of Balkan Science conference of Biology, 2005, p 329-338

Edible vaccine against hepatitis B virus. Gergna Zahmanova, Vesela Radeva, Stefan Dukiandjiev, Ivan Minkov and Andon Andonov; BioTechnology& Technological Equipment 3/2004/18 Supplement p.40;

Investigation of immunogenicity and stability of synthetic HBc 153 protein and WHV153 in human digestive tract. G. Zahmanova, S. Naimov, S. Dukiandjiev, I. Minkov and A. Andonov, Научни трудове на Пловдивски университет – биология. Homo, 2007, 40: 33-40

Храни ваксини – алтернативи срещу хепатит В инфекция. Г. Захманова, В. Радева, И. Минков и С.  Дюкяанджиев; Сборник доклади на петата национална научно-техническа конференция "Екология и здраве". 2004, том 1:399-404

Хепатитния вирус по северноамериканския мармот – система за създаване на рекомбинантни ваксини. Г. Захманова, С. Наимов, М. Костова, С. Дюкянджиев, И. Минков, А. Андонов, Научни трудове на Пловдивски университет – биология. Homo, 2007. 41:131-139

Бърз PCR – базиран метод за получаване на синтетични гени и оптимизация на нуклеотидната последователност с цел оптимизация на експресионните нива при трансформация на растения. С. Наимов, М. Костова, Г. Захманова, Р. Бончева, Ц. Гечев, И. Денев, Г. Яхубян, С. Дюкянджиев, И. Минков, Научни трудове на Пловдивски университет – биология. Plantarum, 2007: 40: 13-18

Стабилност и имуногенност на хибридния инсектициден протеин SN 19 при орално постъпване в храносмилателния тракт на бозайници. С. Наимов, М. Костова, Г. Захманова, Р. Бончева, Ц. Гечев, И. Денев, Г. Яхубян, С. Дюкянджиев, И. Минков. Научни трудове на Пловдивски университет – биология.  Plantarum, 2007: 40: 55-60

Изследване на биоразнообразието на инсектицидните делта-ендотоксини в българска колекция B. Thuringiensis, като средство за контрол на насекомните популации в условията на органично земеделие. С. Наимов, М.  Костова, Г. Захманова, Р.  Бончева, Ц.  Гечев, И. Денев, Г. Яхубян, С. Дюкянджиев, И. Минков. Научни трудове на Пловдивски университет – биология. Plantarum, 2007: 40: 49-54