Contact addresses:

Dr. Iliya D. Denev

Plant Physiology and Molecular Biology Dept.

University of Plovdiv

24 Tsar Assen St.

4000 Plovdiv

BULGARIA

Phone +359 32 261 501 (office) +359 887 453 255 (Cellular)

Emails: iliden@pu.acad.bg or iliya.denev@gmail.com

Education:

Ph.D. Plant Physiology and Plant Biochemistry awarded by Bulgarian Academy of Sciences.

M.S. in Biology: Faculty of Biology, University of Plovdiv

Languages:

Mother tongue: Bulgarian

English and Russian written and spoken in very good command

Employment History:

Present position: Associated professor in molecular biology, Department of Plant Physiology and Molecular Biology, University of Plovdiv, Bulgaria  2007-

Previous positions: Senior lecturer in Department of Plant Physiology and Molecular Biology, University of Plovdiv, Bulgaria 2004 - 2007 (temporary without breaking of my career I was Projects Manager at the Beadle Biotechnology and Genomic Research Center, University of Nebraska-Lincoln)

Postdoctoral researcher in:

-Virginia Polytechnic Institute and State University, Plant Pathology, Physiology and Weed Science Department, Blacksburg, VA, USA 2002-2003

-The Cell cybernetics Unit, Plant Research International, Wageningen, The Netherlands. 1999-2001

Senior lecturer, Department of Plant Physiology and Molecular Biology, University of Plovdiv, Bulgaria appointed - September 1995-1998

Assistant -professor, Department of Plant Physiology, University of Plovdiv, appointed - May 1989-1995

Teaching experience:

Bachelor level: 

(Undergraduate)

Eighteen years teaching experience including following lecture and practical courses:

-Lecture courses and seminars in basic molecular biology, molecular genetics, plant biochemistry, membrane biology.

-Practical courses in molecular biology, molecular genetics, membrane biology, plant biochemistry, electron microscopy, separation methods in biological experiments, isotope techniques in molecular biology

Master level:

(Graduate)

-Lecture and practice in: Molecular plant pathology, Molecular aspects of Photosynthesis, Molecular taxonomy dedicated to graduated students studying in M.Sc. programs "Biotechnology", "Bioinformatics" and "Molecular Biology".

Supervision of students:

Thirty four undergraduate students (B.Sc. level), fifteen graduate students and two Ph.D. student in PU "Paissi Hilendarski", Plovdiv, Bulgaria;

One graduate student in Wageningen Agricultural University, Wageningen, The Netherlands

Three undergraduate in Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA

Scientific training and experience:

Scientific training chronology:

Scientific training chronology:

2006 -

Principal Investigator & Senior Researcher, Plant Physiology and Molecular Biology Dept. University of Plovdiv, Bulgaria

Main goals of research:

-Identification and isolation of genes encoding antioxydant enzymes; =Identification and isolation of genes involved in plant-pathogen interactions and resistance;

-Studying of molecular signaling in Arabidosis;

-Identification and isolation of genes from carotenoid synthetic pathway in sweet pepper (C. annuum L.)

Research expertise:

-Expression in bacterial, yeast (Pichia) and plant tissue culture systems  &Agro-infiltrated plants leaves of various plant proteins

-Purification of the proteins and analyses of protein-protein/protein-lipid and protein-DNA interactions by FPLC, MS-MS Maldi-MS, Biacore2000.

-Special training at Biacore main facilities at Piscataway, NY acquired

-All basic molecular biology techniques from sequence-based cloning &sequencing to expression in different systems

2004-2006

Projects Manager & Senior Researcher, University of Nebraska-Lincoln, Lincoln, USA

Main aim of the research: Expression in bacterial, yeast (Pichia) and plant tissue culture systems  &Agro-infiltrated plants leaves of various plant proteins

Research expertise:

-Purification of the proteins and analyses of protein-protein/protein-lipid and protein-DNA interactions by FPLC, MS-MS Maldi-MS, Biacore2000.

Special training at Biacore main facilities at Piscataway, NY acquired (diploma could presented upon request)

-All basic molecular biology techniques from sequence-based cloning &sequencing to expression in different systems

1999-2003 – Postdoctoral Research "Biosynthesis of germination stimulants for Striga and Orobanche - biochemistry and molecular biology"

Part of the research took place at Virginia Polytechnic Institute and State University, Plant Pathology, Physiology and Weed Science Department, Blacksburg, VA, USA and other part was in The Cell cybernetics Unit, Plant Research International, Wageningen, The Netherlands.

Research was focused on plant-to plant signalling, mechnizms of plant cell walls penetration and membrane fusion during th invasion of parasite into vascular tissues of the host plants

Research training in:

-Screening of large scale Arabidopsis T-DNA knock-out and activation tag mutants

-Analysis of the number and position of inserts by Southern blot and Tail-PCR technique, gene/cDNA sequencing

-Identification, isolation and cloning of tagged genes by use of techniques like Northern blotting, one and two steps RT-PCR; 3'/5'-RACE-PCR, sequential cloning etc.

-Enzymology and biochemistry of isoprenoid synthesis, (including work with radio-labelled substrates for enzymes and their products)

-Analytical techniques like radio-GC, GC-MS, HPLC.

1994–1998 Ph.D. thesis "Membrane organization of protochlorophyllide oxidoreductase in isolated inner membranes from wheat etioplasts and etiochloroplasts, examined by fluorescence probes 1-aniline-8-naphthalene sulfonate and pyrene".

The main part of investigations took place in the group of Prof. Christer Sundqvist, Plant Physiology Dept., University of G?teborg, G?teborg, Sweden. Part was conducted at the Photobiology Institute, Belarusian Academy of Sciences, Minsk, Belarus and another part was done in Department of Plant Physiology and Molecular Biology, University of Plovdiv, Plovdiv, Bulgaria.

-In vitro expression of plasmids with POR genes

-Isolation and analysis of membrane proteins by ion-exchange column chromatography, native and SDS-PAGE;

-Steady state and time resolved fluorescent spectroscopy

-Low temperature fluorescent spectroscopy

-Use of membrane fluorescent probes

-Pigments and lipid isolation and analysis by column chromatography, TLC, HTLC, HPLC

-Preparation of large unilamellar vesicles and building of artificial membrane systems.

Grants and awards:

Levels:

B.S and M.S student at the University of Plovdiv:

Ph.D. student:

Postdoctoral research:

1986 I was granted with personal "Vasil Kolarov's" Scholarship form the Ministry of Education.

1989 I graduated the University of Plovdiv awarded with gold medal from the Ministry of Education.

In 1994 I was granted with 9 months scholarship from the Swedish Institute.

In 1996 and 1997 my work in Sweden was funded from the local EC-TEMPUS project.

1998-1999, and 1999-2000 Individual IAC (International Agricultural Centre - The Netherlands) grants "Biosynthesis of germination stimulant for parasitic weeds".

1999- 2001 - Project leader of NSF-Bulgaria funded project K-812-99  "Use of artificial membrane systems to reveal mechanisms of prolamellar bodies formation ".

2000-2001, Marie Curie Individual Fellowship: "Biosynthesis of germination stimulants for Orobanche (O. ramosa and O. cumana) - biochemistry and molecular biology"

2002-2003 NATO –Individual postdoctoral fellowship: Increasing tobacco resistance against broomrapes (Orobanche spp.) through investigations of key genes in germination signal biosynthesis and host root response to parasitism.

Current research grants:

2003-2005 Project leader of project funded by internal university funds 13-B-15: - Identification of key genes contributing to the increased resistance of important crops, against broomrape weeds (Orobanche spp.)

2004-2005 – Project co-director of NATO-Science for Peace program funded grant LST.CLG.980142 -  Isolation and characterization of key-genes for resistance against Broomrapes (Orobanche spp.)

2003-2006 - Project leader of NSF-Bulgaria funded project K-1305: Increasing the crop resistance against broomrapes (Orobanche spp.) by identification and manipulation of key genes in biosynthetic pathway of Orobanche seed's germination stimulants

2004-2007 – Project leader of NSF-Bulgaria funded project G-402 - Search for genes regulating early steps of interactions between host crops and broomrapes (Orobanhce spp.)

2005-2008 - PI of NSF-Bulgaria funded project B1516 – Isolation and molecular analyses of genes encoding superoxide dusmutase enzymes in Haberlea rhodopensis

2006-2009 PI of NSF-Bulgaria funded project G-501 "Reactive oxygen species as signaling molecules controlling stress adaptation and programmed cell death in plants

2006-2009 - Project leader of NSF-Bulgaria funded project U-602 "Support for investigations directed toward identification of key genes that determine plant resistance against biotic and abiotic stresses in the Genomic Research Center at the University of Plovdiv"

2006-2009 – Project leader of NSF-Bulgaria funded project UB 406 "Study of a mutation in pepper (Capsicum annuum Linn.) related to high beta-carotene content"

References:

1. Dr. Rumen Mladenov – Vice Dean "Teaching and research affairs" – Faculty of Biology, University of Plovdiv, 24 Tsar Assen St. 4000 Plovdiv, Bulgaria, Phone +359 32 261 518, email rummlad@pu.acad.bg This email address is being protected from spam bots, you need Javascript enabled to view it

2. Prof. Rossitza Buchvarova – Agro-Bio-Institute; 8 Dragan Tsankov Str., 1164 Sofia, BULGARIA, Phone +359 2 963 54 09 Fax: +359 2 963 54 08, Email: rossyb@abi.bg This email address is being protected from spam bots, you need Javascript enabled to view it

3. Dr. Detelin Stefanov, Institute of Plant Physiology, Bulgarian Academy of Sciences, 21 Acad. G. Bonchev St, Sofia, Bulgaria, Phone +359 2 979688, email: detelin@obzor.bio21.bas.bg This email address is being protected from spam bots, you need Javascript enabled to view it

4. Prof. Christer Sundqvist, Head of the Department of Plant Physiology, University of Goteborg, Box 461, SE-405 30 Goteborg, Sweden Phone +46 31 773 2602; Fax + 46 31 773 2626; E-mail: christer.sundqvist@fysbot.gu.se This email address is being protected from spam bots, you need Javascript enabled to view it

5. Dr. James H. Westwood, Virginia Polytechnic Institute and State University, Plant Pathology, Physiology and Weed Science, 406 Price Hall, Blacksburg, VA 24061-0331, USA, Phone:+1 540 231 7519, Fax +1 231 540 7477, email: westwood@vt.edu This email address is being protected from spam bots, you need Javascript enabled to view it

Publications:

I have 26 research articles and 11 printed abstracts from international research conferences.

Selected recent publications

Petkova V., Denev I., Porjazov I. 2007, Use of chlorophyll fluorescence parameters in breeding of common bean (Phaseoulus vulgaris L.) for heat-tolerance in field conditions Scientia Horticulture 111/2 , 101-106

Tsanko S. Gechev, Frank Van Breusegem, Julie M. Stone, Iliya D. Denev and Christophe Laloi, 2006 Reactive oxygen species as signals that modulate plant stress responses and programmed cell death, BioEssays 28:1091–1101

Denev I., Yahoubian G., Minkov I., Sundqvist C., 2005 Light-induced changes of protochlorophyllide oxidoreductase organization in prolamellar bodies, isolated from etiolated carotenoid-deficient wheat plants, revealed by fluorescence probes", BBA-Biomembranes, 1716, 97-103

Denev I., Yahubian G., Minkov I. 2005 Recent advances in chloroplast development in higher plants, In Handbook of Photosynthesis, 2nd edition (Pessarakli M ed.) Marsel Dekker Inc., New York , chapter 18. pp 451-467.

Other recent publications

Iliya D. Denev, Bistra E. Deneva, Rossitza Buchvarova, 2007, The biosynthetic origin of germination stimulants for Orobanche ramosa (L.) in tobacco and Arabidopsis., Biotechnology and Biotechnological Equipment, vol 21:1, 54-57.

Denev Illya, Dimitrova Victoria, Naimov Samir, Tsanko Gechev and Yahubyan Galina, 2007, Design of degenerative primers for identification of "putative SOD genes" by PCR, Trav. Sci. Univ. Plovdiv, Plantarum, 40:6, 31-38

Denev Iliya., Nilsson Ralf2, Yahubyan Galina,  Tsanko Gechev, Naimov Samir, Liljenberg Conny2 2007, Lipid composition of cell plasma membrane and drought stress – Study in model membrane system, Trav. Sci. Univ. Plovdiv, Plantarum, 40:6, 19-30

Mishev K., Denev I., Radeva G., Ananiev E. D. 2006, RNA transcription in isolated chloroplasts during senescence and rejuvenation of intact cotyledons of Cucurbita  pepo  L.  (Zucchini), Compt Rend Bulg Acad Sci –Biologie (Physiologie des plantes) , 59, 1287 – 1292.

Denev I., Yahubyan G., , Stefanov D., (2005) Changes in chlorophyll pigments in leaves of Haberlea rhodopensis (Friv) grown under continuous darkness, Proceeding of Plovdiv Unviversity, 15, 157-161

Denev I., Yahubyan G., and Stefanov D. (2005), Synthesis of chlorophyll in leaves of Haberlea rhodopensis (Friv.) during prolonged darkening. Compt Rend Bulg Acad Sci –Biologie (Physiologie des plantes) , 58, 1429 – 1432.

I. Gadjev, I. Denev, V. Radeva, .I. Minkov, J. Hille, T. Gechev (2005) Molecular biology and genetics of programmed cell death in Arabidopsis thaliana, Proceeding of Plovdiv Unviversity, 15, 231–237

Yahubyan G., Denev I., Gozmanova M., (2005) Determination of the multiple izoforms of some antioxidant enzymes in Haberlea rhodopensis, Proceeding of Plovdiv Unviversity, 15, 226-230.

Articles in press:

Denev I., Stefanov D., Yahubyan G., Minkov I, Terashima I., Delayed dark induced senescence in homoiochlorophyllous Balkan endemic plant Haberlea rhodopensis Friv., Functional Plant Biol. submitted

Denev I., Atanasova S., Buchvarova R., "Application of T-DNA insertion mutagenesis in search of genes for resistance against Broomrapes (Orobanche spp.), Weed research submitted

Yahubyan G., Gozmanova M., Denev I., Minkov I., Antioxidative potential of the resurrection plant Haberlea rhodopensis, J. Plant Physiol. submitted

Denev I., Hellgren L., Sundqvist C., Light-induced changes of dynamic properties and organization of lipids in isolated inner etioplast membranes revealed by Laurdan fluorescence. BBA submitted.