Family name: Gozmanova
First names: Mariyana Hristova
Place of birth: Vratsa (Bulgaria)
Date of birth: June 25, 1973
Plovdiv University "Paisii Hilendarski"
Dept. of Plant Physiology and Molecular Biology
24, Tsar Assen St.
Work telephone: +359 32 629 495
Work fax: +329 32 629 595
July 1991: Diploma Excellent (6.00/6.00) at "Hristo Botev" High Scholl Vratsa, Bulgaria
Oct 1991-July1996: Faculty of Biology, University of Plovdiv
July 1996: Graduated in Biology with the Thesis "Investigation of SAN-9897 influence on the structure and function of PLB by measure with fluorescent probe 1.8 ANS and pyren".
February 1997-Research Assistant in the Dept. of Plant Physiology and February2000 Molecular Biology, Plovdiv University
February 2000: Admitted to the PhD course in Molecular Biology of Plovdiv University, Faculty of Biology by passing a written and oral examination on molecular biology and scientific English.
September 2000: Prepared a monographic study on "Molecular biology of RNA" in context of the PhD course.
November 2000: Marie Curie Training Fellowship, awarded for practical
November 2001: Training in the Institute of Molecular Biology and Biotechnology, Heraklion, Greece.
February 2002: Fellowship of Greek State Scholarships Foundation IKY,
February 2003: Awarded for research in Faculty of Biology, University of Crete, Heraklion, Greece.
August 2002: Candidate for the European Doctorate in BioTechnology
November 2003: Assistant Professor, Dept. of Plant Physiology and Molecular Biology, Plovdiv University
Mariyana Gozmanova, Michela Alessandra Denti, Ivan Nikiforov Minkov, Mina Tsagris, Martin Tabler "Characterization of the RNA motif responsible for the specific interaction of potato spindle tuber viroid RNA (PSTVd) and the tomato protein Virp1", Nucleic Acids Research, 2003, Vol.31, No.19, 5534-5543
Participation at meetings and courses:
April 9-12. 2001 - Attended ICGEB Theoretical course "RNA Structure and Function", Trieste, Italy
September 1-6. 2002 - 13 FESPP Congress, Heraklion, Crete - Poster Session: "Biotic stress"- Abstract "The motif in the Terminal Right Part of Potato Spindle Tuber Viroid is the determinant of specific in vitro recognition by tomato RNA- binding protein Virp1"
October 27-31. 2002 - EPSO conference 2002, Brunnen, Switzerland- Poster session: "Basic Biological Processes"- Abstract "Identification of the RNA binding domain of Virp-1"
March 29 - Attended Advance Course in Biotechnology Ethics,
April 4, 2003 Versailles, France
November 19-21, 2003 - X Annual conference of Biology Faculty, Sofia University, Sofia Poster session: "Molecular Biology"- Abstract "71 nucleotides from the terminal right part of PSTVd is the sufficient minimum of RNA capable of sustaining specific binding to tomato protein Vipr1"
Jan.11-14, 2004 - International Quality Network (winter meeting) "Biochemistry of Nucleic Acids", Oral presentation "Characterization of the RNA motif responsible for the specific interaction of potato spindle tuber viroid RNA (PSTVd) and the tomato protein Virp1", Justus-Liebig University Giessen, Germany
June, 21, 2004 - Public defense of the PhD thesis, Sofia, Scientific council "Molecular biology, Biophysics and Biochemistry"
Sept.13-18,2004 - International training workshop on Bioinformatics, Heraklion, Crete, organized by University if Crete, IMBB and with the support of the Unesco Venice Office.
Sept. 23-24, 2004 - Days of Virology in Bulgaria with International participation, Sofia, Bulgaria Poster session: Plant Virology- Abstract "Specificity of PSTVd RNA and viroid-binding protein (Virp1) interaction: a role of the auxiliary domains of Virp1 upon the RNA activity"
Technical experience in molecular biology:
• basic techniques (gel electrophoresis of nucleic acids, nucleic acid radiolabelling (32P), hybridisation, etc.);
• basic techniques for DNA manipulation (somatic DNA preparation, Southern blots, molecular cloning and subcloning, restriction map analysis, DNA sequencing);
• basic techniques for RNA molecular biology (RNA extraction, Northern blot, in vitro transcription);
• basic techniques for protein molecular biology (SDS- PAGE of proteins, Western blots, fusion proteins expression);
• computer methods for molecular biology (software and on-line packages for DNA and protein sequence analysis, database similarity searches, RNA structure prediction);
• Polymerase Chain Reaction (PCR) and RT PCR;
• Electrophoresis mobility shift assays (EMSA) and competition experiments with RNA-binding proteins;
• North-Western assays;
• Mother tongue: Bulgarian
• Spoken and written English at advanced levels
• Spoken and written Russian at intermediate levels